Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14856
Título: | A fluorescence-LC method with NDA pre-column derivatization for fumonisin B2 determination in black aspergilli cultures |
Autor(es): | Abrunhosa, Luís Calado, Thalita Resende, Tiago Venâncio, Armando |
Palavras-chave: | Aspergillus niger Fumonisin B2 black aspergilli fluorescence detection HPLC Naphthalene-2,3-dicarboxaldehyde |
Data: | Out-2011 |
Editora: | Taylor & Francis |
Revista: | Journal of Liquid Chromatography & Related Technologies |
Resumo(s): | The recent discovery that Aspergillus niger can produce fumonisins raises a new mycotoxigenic risk that requires additional studies to assess the impact on agricultural commodities. Here, we report the development and validation of a sensitive LC-fluorescence method for the identification of fumonisin B2 in black aspergilli cultures. The performances of naphthalene-2,3-- dicarboxaldehyde (NDA), o-phthaldialdehyde (OPA), and 9-fluorenylmethyl chloroformate (FMOC) for the precolumn derivatization of samples were evaluated and compared. The best methodology included the following elements: NDA was used during pre-column derivatization; the derivatives were separated by a 30min isocratic elution with acetonitrile=H2O=acetic acid (60:40:1, v=v=v), pumped at a flow rate of 1mL min 1 on a polar C18 reversed-phase YMC-Pack ODS-AQ column (250 4.6mm, 5 lm); and fluorescence detection using excitation and emission wavelengths of 420 and 500 nm, respectively. The calibration curve was found to give a good linear response (r2>0.9999) from 1 to 200 lg L 1. For FB2, which is the main fumonisin detected in black aspergilli thus far, this method measured an LOD and an LOQ of 2 and 6 lg kg 1, respectively. Using this method, the amount of FB2 in black aspergilli cultures can be simply and rapidly determined. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/14856 |
DOI: | 10.1080/10826076.2011.575982 |
ISSN: | 1520-572X 1082-6076 |
Versão da editora: | http://pubs.acs.org/doi/abs/10.1021/jf202123q |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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abrunhosa_etal_2011_JLCRT.pdf Acesso restrito! | documento principal | 289,58 kB | Adobe PDF | Ver/Abrir |