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https://hdl.handle.net/1822/31455
Título: | Production and characterization of protease from Penicillium aurantiogriseum URM 4622 |
Autor(es): | Rodrigues, Priscila M. B. Lima, Carolina A. Filho, José Luiz L. Teixeira, J. A. Porto, Ana L. F. Cunha, Maria G. Carneiro da |
Palavras-chave: | Penicillium aurantiogriseum Production Characterization Protease Bacth Bioreactor Detergent |
Data: | 2008 |
Citação: | Rodrigues, Priscila M. B.; Lima, Carolina; Filho, J. L. Lima; Teixeira, José A.; Porto, A. L. F.; Carneiro-da-Cunha, M. G., Production and characterization of protease from Penicillium aurantiogriseum URM 4622. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 97-104 |
Resumo(s): | Proteases with new properties are required due to their increasing industrial importance. In this work, the optimal fermentation conditions for the production of a protease from Penicillium aurantiogriseum dierchx (URM-4622) are presented together with partial characterization of the protease catalytic properties. The batch fermentation conditions that allow for the highest specific proteolytic activity are 26 ºC, pH 7.0, and 25 % saturation dissolved O2 concentration. The obtained protease is stable over a wide range of pH (5.8 to 9.5) and temperature (25 to 40 ºC) values. In the presence of Zn2+ a 26 % reduction in the enzyme proteolytic activity occurs and, in contrast, Mn2+ enhances its activity by 28.9 %. 96.2 % and 70.8 % of the protease activity are maintained after 90 min incubation in 5 and 10 % (v/v) H2O2 aqueous solutions, respectively. PMSF inhibition reveals that this enzyme is a serine protease. Protease is able to hydrolyze different proteins. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/31455 |
ISBN: | 978-972-97810-5-6 |
Acesso: | Acesso aberto |
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