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https://hdl.handle.net/1822/65111
Título: | Sweet Cherry (Prunus avium L.) PaPIP1;4 is a functional aquaporin upregulated by pre-harvest calcium treatments that prevent cracking |
Autor(es): | Breia, Richard Maykel Gonçalves Mósca, Andreia F. Conde, Artur Correia, Sofia Conde, Carlos Noronha, Henrique Soveral, Graça Gonçalves, Berta Gerós, H. |
Palavras-chave: | aquaporins PaPIP1 4 calcium application fruit-cracking sweet-cherry PaPIP1;4 |
Data: | 24-Abr-2020 |
Editora: | MDPI |
Revista: | International Journal of Molecular Sciences |
Citação: | Breia, Richard; Mósca, Andreia F.; Conde, Artur; Correia, Sofia; Conde, Carlos; Noronha, Henrique; Soveral, Graça; Gonçalves, Berta; Gerós, Hernâni, Sweet Cherry (Prunus avium L.) PaPIP1;4 Is a functional aquaporin upregulated by pre-harvest calcium treatments that prevent cracking. International Journal of Molecular Sciences, 21(8), 3017, 2020 |
Resumo(s): | The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl2, which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl2-treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H2O2 that is upregulated by the pre-harvest exogenous application of CaCl2 supplements. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65111 |
ISBN: | 1424-6783 |
DOI: | 10.3390/ijms21083017 |
ISSN: | 1661-6596 |
e-ISSN: | 1661-6596 |
Versão da editora: | https://www.mdpi.com/1422-0067/21/8/3017 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53648_1.pdf | 3,26 MB | Adobe PDF | Ver/Abrir |