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https://hdl.handle.net/1822/74500
Título: | Monitoring bacteriophage infection on bacterial cells using FISH |
Autor(es): | Vilas Boas, Diana Melo, Luís Daniel Rodrigues |
Palavras-chave: | Bacteriophage Detection Microscopy Phage-host interactions |
Data: | 13-Fev-2021 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Vilas Boas, Diana; Melo, Luís Daniel Rodrigues, Monitoring bacteriophage infection on bacterial cells using FISH. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 157-168 |
Resumo(s): | The possibility of visualizing bacteriophage-host interactions through fluorescence in situ hybridization (FISH)-derived methods is gaining relevance in the last few years. These methods allow the possibility of discriminating between phage-infected and noninfected cells and the assessment of the different infection stages at the single cell level. In opposition to bacterial cells, the detection of phages is more challenging due to the low number of nucleic acid copies. However, by using a conserved region of the phage genome that is highly expressed during transcription, a FISH signal targeting phage DNA copies and mRNA transcripts can be easily visible inside the bacterial host cells. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/74500 |
ISBN: | 978-1-0716-1115-9 |
DOI: | 10.1007/978-1-0716-1115-9_11 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/book/10.1007/978-1-0716-1115-9 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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document_54197_1.pdf Acesso restrito! | 213,39 kB | Adobe PDF | Ver/Abrir |