Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75676
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Campo DC | Valor | Idioma |
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dc.contributor.author | Freitas, Ana Isabel | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Aguiar, Tatiana Quinta | por |
dc.date.accessioned | 2022-01-25T17:34:49Z | - |
dc.date.available | 2022-01-25T17:34:49Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Q., Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials. Journal of Advanced Research, 36, 249-264, 2022 | por |
dc.identifier.issn | 2090-1232 | por |
dc.identifier.uri | https://hdl.handle.net/1822/75676 | - |
dc.description.abstract | Background The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization of recombinant proteins/peptides onto/into natural, synthetic or hybrid materials in a single-step are arising and attracting increasing interest. Aim of Review This review highlights the most significant advances of the last 5 years within the scope of tag-mediated protein purification/immobilization and elucidates their contributions for the development of efficient single-step purification and immobilization strategies. Recent progresses in the field of protein-engineered materials created using innovative protein-tag combinations and future opportunities created by these new technologies are also summarized and identified herein. Key Scientific Concepts of Review Protein purification/immobilization tags present a remarkable ability to establish specific non-covalent/covalent interactions between solid materials and biological elements, which prompted the creation of tailor-made and advanced functional materials, and of next-generation hybrid materials. Affinity tags can bind to a wide range of materials (of synthetic, natural or hybrid nature), being most suitable for protein purification. Covalently binding tags are most suitable for long-term protein immobilization, but can only bind naturally to protein-based materials. Hybrid affinity-covalently binding tags have allowed efficient one-step purification and immobilization of proteins onto different materials, as well as the development of innovative protein-engineered materials. Self-aggregating tags have been particularly useful in combination with other tags for generating protein-engineered materials with self-assembling, flexible and/or responsive properties. While these tags have been mainly explored for independent protein purification, immobilization or functionalization purposes, efficient strategies that combine tag-mediated purification and immobilization/functionalization in a single-step will be essential to guarantee the sustainable manufacturing of advanced protein-engineered materials. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, Project EcoBioInks4SmartTextiles [PTDC/CTM-TEX/30298/2017–POCI-01-0145-FEDER-030298], co-funded by the European Regional Development Fund (ERDF) through the Competitiveness and Internationalization Operational Programme (COMPETE 2020) of Portugal 2020, and BioTecNorte operation [NORTE-01-0145-FEDER-000004], funded by the ERDF under the scope of Norte2020 - North Region Operational Programme. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | PTDC/CTM-TEX/30298/2017 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | Protein tags | por |
dc.subject | Solid-binding peptides | por |
dc.subject | Non-covalent binding | por |
dc.subject | Covalent binding | por |
dc.subject | Protein-engineered materials | por |
dc.subject | Protein purification/immobilization | por |
dc.title | Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/journal/journal-of-advanced-research | por |
dc.comments | CEB54479 | por |
oaire.citationStartPage | 249 | por |
oaire.citationEndPage | 264 | por |
oaire.citationConferencePlace | Egypt | - |
oaire.citationVolume | 36 | por |
dc.date.updated | 2022-01-25T16:59:55Z | - |
dc.identifier.doi | 10.1016/j.jare.2021.06.010 | por |
dc.identifier.pmid | 35127175 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Advanced Research | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54479_1.pdf | 1,43 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons