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https://hdl.handle.net/1822/83902
Título: | Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
Autor(es): | Azinheiro, Sarah Rodríguez-López, Pedro Lozano-León, Antonio Guedes, Hugo Regal, Patricia Franco, Carlos M. Cepeda, Alberto Teixeira, Maria Pilar Araújo Melo, Luís Daniel Rodrigues Silva, Daniela Fernández, Ana Faria, Márcia Roumani, Foteini Juan Herrera, Prado, Marta López-Cabo, Marta Garrido-Maestu, Alejandro |
Palavras-chave: | Interlaboratory validation qPCR Ready-to-eat Fish products Alternative methods Listeria monocytogenes |
Data: | Ago-2023 |
Editora: | Elsevier 1 |
Revista: | Food Control |
Citação: | Azinheiro, Sarah; Rodríguez-López, Pedro; Lozano-León, Antonio; Guedes, Hugo; Regal, Patricia; Franco, Carlos M.; Cepeda, Alberto; Teixeira, Pilar; Melo, Luís Daniel Rodrigues; Silva, Daniela; Fernández, Ana; Faria, Márcia; Roumani, Foteini; Herrera, Juan; Prado, Marta; López-Cabo, Marta; Garrido-Maestu, Alejandro, Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product. Food Control, 150(109769), 2023 |
Resumo(s): | Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/83902 |
DOI: | 10.1016/j.foodcont.2023.109769 |
ISSN: | 0956-7135 |
Versão da editora: | http://www.journals.elsevier.com/food-control |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56150_1.pdf | 2,44 MB | Adobe PDF | Ver/Abrir |