Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/89972
Título: | Development of a new technology to detect respiratory infectious diseases |
Autor(es): | Nascimento, A. Abreu, C. Silva, M. Leite, B. Rouly, S. Abreu, Maria José Ferraz, R. Costa, M. Prudêncio, Parente, E. Vieira, M. |
Palavras-chave: | Face mask Biosensor SARS-CoV-2 virus Sialic acid |
Data: | 1-Dez-2022 |
Revista: | inSTEMM Journal |
Citação: | Vieira, M., Nascimento, A. M., Abreu, C., Leite, B. ., Silva, M., Abreu, M. J., Ferraz, R. ., Rouly, S. ., Parente, E. ., Costa, M. ., & Prudêncio, C. (2022). Development of a new technology to detect respiratory infectious diseases. InSTEMM Journal, 1(S2). https://doi.org/10.56725/instemm.v1iS2.13 |
Resumo(s): | The Covid-19 pandemic brought the need to use social masks to prevent the spread of the SARS-CoV-2 virus. However, no reliable and fast method were yet established to detect viral particles and to improve the protective ability of social masks. Through color changes, colorimetric biosensors can be used as a rapid and easily approach to detect virus. Gold nanoparticles (AuNP) are known to have excellent optical properties and huge research potential. The new SARS-CoV-2 has the ability of entering human body cells, namely through a second pathway of entry – the sialic acid (SA) receptor. In order to respond to the emergency and to contribute to the diminishing of the spread of SAR-CoV-2, we developed a colorimetric biosensor based on the functionalization AuNP by sialic acid (SA) (SA-AuNP), as a new and effective textile coating layer, to provide a direct indication of the protective capacity of social masks. To do that, AuNPs (10 nm) were functionalized with SA (SA-AuNP), in three different concentrations (50-50, 30-70 and 20-80, respectively) to select the optimal concentration for respiratory virus detection. Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscope with a Transmission Detector (STEM) analyses confirmed SA-AuNPs binding. FTIR results showed a well-established bond, through matches of peaks of SA-AuNPs. Bindings between the compounds were more evident in 50-50 concentration of SA-AuNP. In the 30-70 SA-AuNP the STEM images show some superposition of the nanoparticles and not so evident binding, as in the 20-80 concentration. Still, between these last two concentrations, the 30-70 is the one that shows the best results since it is visible some circular points larger than the others. To achieve the goal, the concentrations 30-70 and 50-50 of SA-AuNP were impregnated (Textile Foulard) in two substrates of different compositions, a cellulosic and a synthetic one. However, in this technique there are many parameters, such as drying time and temperature, which were varied to understand which the best procedure was to obtain the biosensor. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/89972 |
DOI: | 10.56725/instemm.v1iS2.13 |
ISSN: | 2753-6939 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Instmm Journal View of Development of a sustainable biosensor to detect respiratory infectious diseases.pdf | 5,13 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons