Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/48383
Título: | Molecular analysis of c-Kit and PDGFRA in GISTs diagnosed by EUS |
Autor(es): | Gomes, Ana L. Bardales, Ricardo H. Milanezi, Maria Fernanda Grillo Reis, R. M. Schmitt, Fernando |
Palavras-chave: | Endoscopic ultrasound-guided fine-needle aspiration c-Kit Platelet-derived growth factor-α PDGFRA Gastrointestinal stromal tumor GIST Mutations |
Data: | 2007 |
Editora: | American Society for Clinical Pathologists (ASCP) |
Revista: | American Journal of Clinical Pathology |
Resumo(s): | Gastrointestinal stromal tumors (GISTs) are characterized by overexpression and mutations of c-Kit. Approximately 80% of c-Kit mutations occur in exon H, being a response factor to imatinib (Gleevec) therapy. Mutations of platelet-derived growth factor receptor-a (PDGFRA) are observed in a subset of GISTs lacking c-Kit mutations. We aimed to assess whether c-Kit and PDGFRA mutation analysis of GISTs obtained by endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) could be routinely performed. Mutation analysis of c-Kit hotspot exons (9, 11, 13, and 17) and PDGFRA hotspot exons (12 and 18) was performed in aspirates of 33 GISTs and 18 non-GIST mesenchymal tumors. Of the GIST cases, 19 (58%) of 33 contained a mutation in exon 11, 1 (3%) in exon 9, and none in exons 13 and 17. No activating c-Kit mutations were identified in non-GIST cases. No PDGFRA mutation was detected. Mutation analysis is possible in these FNA cell blocks and can assist in the diagnosis and therapeutic decisions in GIST cases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/48383 |
DOI: | 10.1309/M1EC8JE9ACAMJACU |
ISSN: | 1943-7722 |
e-ISSN: | 0002-9173 |
Versão da editora: | http://ajcp.ascpjournals.org/content/127/1/89.long |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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gomes al_am j clin pathol 2007.pdf | 184,46 kB | Adobe PDF | Ver/Abrir |