Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75676
Título: | Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials |
Autor(es): | Freitas, Ana Isabel Domingues, Lucília Aguiar, Tatiana Quinta |
Palavras-chave: | Protein tags Solid-binding peptides Non-covalent binding Covalent binding Protein-engineered materials Protein purification/immobilization |
Data: | 2022 |
Editora: | Elsevier 1 |
Revista: | Journal of Advanced Research |
Citação: | Freitas, Ana Isabel; Domingues, Lucília; Aguiar, Tatiana Q., Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials. Journal of Advanced Research, 36, 249-264, 2022 |
Resumo(s): | Background The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization of recombinant proteins/peptides onto/into natural, synthetic or hybrid materials in a single-step are arising and attracting increasing interest. Aim of Review This review highlights the most significant advances of the last 5 years within the scope of tag-mediated protein purification/immobilization and elucidates their contributions for the development of efficient single-step purification and immobilization strategies. Recent progresses in the field of protein-engineered materials created using innovative protein-tag combinations and future opportunities created by these new technologies are also summarized and identified herein. Key Scientific Concepts of Review Protein purification/immobilization tags present a remarkable ability to establish specific non-covalent/covalent interactions between solid materials and biological elements, which prompted the creation of tailor-made and advanced functional materials, and of next-generation hybrid materials. Affinity tags can bind to a wide range of materials (of synthetic, natural or hybrid nature), being most suitable for protein purification. Covalently binding tags are most suitable for long-term protein immobilization, but can only bind naturally to protein-based materials. Hybrid affinity-covalently binding tags have allowed efficient one-step purification and immobilization of proteins onto different materials, as well as the development of innovative protein-engineered materials. Self-aggregating tags have been particularly useful in combination with other tags for generating protein-engineered materials with self-assembling, flexible and/or responsive properties. While these tags have been mainly explored for independent protein purification, immobilization or functionalization purposes, efficient strategies that combine tag-mediated purification and immobilization/functionalization in a single-step will be essential to guarantee the sustainable manufacturing of advanced protein-engineered materials. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75676 |
DOI: | 10.1016/j.jare.2021.06.010 |
ISSN: | 2090-1232 |
Versão da editora: | https://www.sciencedirect.com/journal/journal-of-advanced-research |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54479_1.pdf | 1,43 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons